Publicación

Tipo de publicación: Artículos científicos (Publicados en revistas de divulgación o científicas)

Título del artículo: Association of the polymorphisms rs179008 (TLR7), rs2004640 (IRF5), rs1800795 (IL-6) and rs2280788 (CCL5) with systemic lupus erythematosus in women of Mayan ethnicity from Yucatan

Registrado por: Nucleosides Nucleotides & Nucleic Acids

Año de publicación: 2024

Resumen: Introduction: IFN-? is the main cytokine in SLE, and single nucleotide polymorphisms (SNP) in different genes could induce it. Aim: To determine the association of rs2004640 (IRF5), rs179008 (TLR7), rs1800795 (IL-6) and rs2280788 (CCL5) with SLE in Mexican women with Mayan ethnicity. Methods: DNA and RNA were isolated from the peripheral blood of 110 patients and 200 healthy control subjects. SNP genotyping and gene expression analysis of IRF5, TLR7, IL-6 and IFN-? were determined by real-time PCR and analyzed with SNP Stat, Stata 10.1 and Graph Pad Prism v5. Results: rs2004640, rs179008, and rs1800795 in both groups were according to Hardy- Weinberg equilibrium. Risk alleles rs179008T and rs2004640T frequencies were higher in controls (p = 0.015 and p = 0.028, respectively), whereas rs179008A frequency was higher in patients (p = 0.015). Allelic combination AGT frequency was higher in patients (p = 0.001). IL-6 rs1800795C > G and CCL5 rs2280788G > C frequencies did not show significant differences (p > 0.05), being rs2280788G (CCL5) monomorphic in controls. SLE patients showed higher TLR7, IRF5, IL6, and IFN-? mRNA levels. IRF5 expression was higher in SLE patients homozygous for rs2004640T (IRF5). Conclusion: This work showed the contribution of TLR7 and IRF5 in SLE pathogenesis in Mayan females from Yucatan.

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Autor(es): Yumi Elena Nakazawa-Ueji, Guillermo Valencia-Pacheco, Lizbeth JosefinaGonzález-Herrera, Laureano Chan-Chalé, Rubí Misol-Ha Velasco-Cárdenas,Martha Julia Carreño-Gonzalez, Gerardo Pérez-Mendoza, Guadalupe IsabelAmaro-Adrián, Edwin Arturo Rodríguez-Dzul, Irma Guadalupe Quintal-Ortiz,Angélica Vanesa Angulo-Ramírez & Ricardo Francisco López-Villanueva

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Número de registro: DOI:10.1080/15257770.2024.2325438